Cloning of Notl-linked DNA Detected by Restriction Landmark Genomic Scanning of Human Genome

Epigenetic alterations are common features of human solid tumors, though global DNA methylation has been difficult to assess. Restriction Landmark Genomic Scanning (RLGS) is one of technology to examine epigenetic alterations at several thousand Notl sites of promoter regions in tumor genome. To assess sequence information for Notl sequences in RLGS gel, we cloned 1,161 unique Notl-linked clones, compromising about 60% of the spots in the soluble region of RLGS profile, and performed BLAT searches on the UCSC genome server, May 2004 Freeze. 1,023 (88%) unique sequences were matched to the CpG islands of human genome showing a large bias of RLGS toward identifying potential genes or CpG islands. The cloned Notl-loci had a high frequency (71%) of occurrence within CpG islands near the 5' ends of known genes rather than within CpG islands near the 3' ends or intragenic regions, making RLGS a potent tool for the identification of gene-associated methylation events. By mixing RLGS gels with all Notl-linked clones, we addressed 151 Notl sequences onto a standard RLGS gel and compared them with previous reports from several types of tumors. We hope our sequence information will be useful to identify novel epigenetic targets in any types of tumor genome.

Role of gamma delta T lymphocyte on Allergic Reaction and Airway Hyper-Reactivity in Mouse Model of Allergy / 소아알레르기및호흡기학회지

No abstract available.

Clinical Characteristics in Patients with Kawasaki Disease Who Received Intravenous Gamma-globulin Retreatment

PURPOSE: Although the use of intravenous gamma-globulin(IVGG) in Kawasaki disease(KD) is effective in reducing clinical symptoms and coronary artery complications, 20-30% of patients have persistent or recrudescent fever and ongoing clinical symptoms. In these patients, the additional infusion of IVGG is considered. The authors studied the characteristics of patients who received IVGG retreatment, and compared them with the patients who did not need IVGG retreatment, for determination of IVGG retreatment. METHODS: We reviewed the medical records of 117 KD patients who could be followed up at least six months. We studied the conventional laboratory findings, electrocardiogram(EKG), signal averaged ECG(SAECG) and echocardiogram. RESULTS: Twenty three patients had early cardiac complications during the six months of follow-up. Four patients had late cardiac complications after six months. The early cardiac complication rate was higher in the IVGG retreatment group than the single infusion group(P<0.0001). The late complication rate was also higher in the retreatment group(P<0.0001). The patients who received methyl-prednisolone(m-PD) pulse therapy had much higher rates of early and late cardiac complications than those who received a single IVGG infusion. Among the clinical data and laboratory findings, only CRP increased significantly in patients who have had the cardiac complications. The IVGG retreatment group had increased CRP than the single infusion group. CONCLUSION: The patients with increased initial CRP may have an increased incidence of complications and an increased possibility of IVGG retreatment. We thought that retreated KD patients might have inflammations severe enough to need high dose IVGG as shown by high CRP levels, and IVGG retreatment could not prevent coronary artery lesions sufficiently.

Clinical Observations On Juvenile Rheumatoid Arthritis: I. Systemic Type / 대한류마티스학회지

OBJECTIVE: To investigate the cl'mical manifestations and laboratory findings observed from the patients with systemic juvenile rheumatoid arthritis. METHODS: Twenty three patients with systemic JRA who were diagnosed and treated at the Department of Pediatrics, Seoul National University Children's Hospital from 1988 to 1993 were investigated for their clinical manifestations and laboratory findings. RESULTS: 1) Boys were affected more prevalently than girls, as a ratio of 2:1. 2) Sixty seven percent of patients were 3 to 6 years old at onset. 3) The clinical manifestations observed were high fever(100%), rash(78%), arthritis(96%), lymphadenopathy(45%), hepatomegaly(41%), splenomegaly(30%), pericarditis(22%), pleuritis(22%), peritonitis(13%) and myalgia(100%). Arthritis occurred with the onset of systemic manifestations in 82% of patients and occurred 8 month later 3 cases in but in one case arthritis proceeded the systemic manifestations for 5 months. The arthritis mainly involved the large joints, knees(68%), ankles(59%), wrists(50%), elbows(32%) and shoulders(23%) and also involved small joints, fingers, (27%) and toes(5%). The number of joints involved was four or fewer in 50% of patients and 5 or more in 50% of patients. The patterns of joint involvement were symmetric(77%) or asymmetric(23%). 4) The main laboratory findings observed were anemia(61%), leukocytosis(91%), thrombocytosis(65%), increased ESR(100%), positive CRP(100%), positive RF(13%) and positive ANA(9%), homogenous or speckled type. One patients gave the transient proteinuria. The histology of lymph nodes showed the follicular hyperplasia of interfollicular plasmocytosis, indicating the nonspecific reactive hyperplasia. The bone marrow showed granulocytosis, monocytosis or toxic granules. CONCLUSION: These data showed the clinical manifestations and laboratory findings of systemic juvenile rheumatoid arthritis in the Korean patients.